Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: EH-P002A promotes wound healing in a dose-dependent manner . A. Molecular structure of EH-P002A. B. No detectable cytotoxicity was observed in HFF-1 or Beas-2B cells upon EH-P002A treatment. C. Wound images from mice treated with EH-P002A at two doses, captured at different time points. D. Wound healing rates on Days 7 and 9. Dose-dependent efficacy on Day 7 was observed.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Dose-dependent cyto-impedance induced by EH-P002A . A . Wound-healing assay images of HFF-1 cells treated with indicated concentrations of EH-P002A, followed by quantification in the right panel. A promoting effect on wound healing was observed at 8 μM, while overdosage of EH-P002A suppressed the migratory ability. B . Images of migrated HFF-1 cells in transwell assays treated with varying EH-P002A concentrations followed by quantification in the right panel, showing promotion at 8 μM and suppression at higher doses. C. The data of wound-healing assay of Beas-2B cells were quantified, showing a promoting effect at 8–20 μM concentrations, and a suppressive effect at a higher concentration of 80 μM. D . Quantification of the transwell assays of Beas-2B cells confirmed dose-dependent cyto-impedance at 40 and 80 μM concentrations. ∗, P < 0.05; ∗∗, P = 0.001; ∗∗∗, P < 0.001.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Wound Healing Assay, Concentration Assay